Boronic Acid-Mediated Activity Control of Split 10–23 DNAzymes

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Abstract

The 10–23 DNAzyme is an artificially developed Mg2+-dependent catalytic oligonucleotide that can cleave an RNA substrate in a sequence-specific fashion. In this study, new split 10–23 DNAzymes made of two nonfunctional fragments, one of which carries a boronic acid group at its 5′ end, while the other has a ribonucleotide at its 3′ end, were designed. Herein it is demonstrated that the addition of Mg2+ ions leads to assembly of the fragments, which in turn induces the formation of a new boronate internucleoside linkage that restores the DNAzyme activity. A systematic evaluation identified the best-performing system. The results highlight key features for efficient control of DNAzyme activity through the formation of boronate linkages.

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Debiais, M., Lelievre, A., Vasseur, J. J., Müller, S., & Smietana, M. (2021). Boronic Acid-Mediated Activity Control of Split 10–23 DNAzymes. Chemistry - A European Journal, 27(3), 1138–1144. https://doi.org/10.1002/chem.202004227

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