Chemically defined medium for oral microorganisms

Abstract

We formulated a chemically defined medium consisting of 14 inorganic salts, 23 amino acids, 23 vitamins and other factors, seven purines and pyrimidines, and glucose which would successfully support the growth of a wide variety of oral microorganisms. Of 204 oral isolates representing 20 genera and 60 species, 197 maintained viability through six serial transfers.