Membrane proteins (MPs) play key roles in most important cellular processes ranging from cell-to-cell communication to signaling processes. Despite recent improvements, the expression of functionally folded MPs in sufficient amounts for functional and structural characterization remains a challenge. Indeed, it is still difficult to predict whether a protein can be overproduced in a functional state in some expression system(s), although studies of high-throughput screens have been issued in recent years. Prokaryotic expression systems present several advantages over eukaryotic ones. Among them, Lactococcus lactis (L. lactis) has emerged in the past years as a good alternative expression system toEscherichia coli. The purpose of this chapter is to describeL. lactis and its tightly inducible system, nisin-controlled gene expression (NICE), for the expression of MPs from both prokaryotic and eukaryotic origins. Moreover, we will describe the functional characterization of some MPs produced in L. lactis.
CITATION STYLE
Bakari, S., André, F., Seigneurin-Berny, D., Delaforge, M., Rolland, N., & Frelet-Barrand, A. (2014). Lactococcus lactis: Recent developments in functional expression of membrane proteins. In Membrane Proteins Production for Structural Analysis (pp. 107–132). Springer New York. https://doi.org/10.1007/978-1-4939-0662-8_5
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