Live imaging using various microscopic technologies is an indispensable tool for investigating the dynamic nature of immune cells. One of the most powerful techniques is the two-photon laser-scanning microscopy (TP-LSM), which has various advantages in observing deep tissues in vivo. Interstitial T cell migration in the lymph node (LN) is a phenomenon intensively examined using TP-LSM in the field of immunology. Intravital and explant methods have been standards for imaging T cell behaviors in the LN, though there are several limitations. Live imaging of LN slices, an LN explant sliced by a vibratome to expose tissue parenchyma, could provide an alternative approach with technical advantages for an in-depth analysis of interstitial T cell migration in vivo.
CITATION STYLE
Katakai, T. (2018). Live imaging of interstitial T cell migration using lymph node slices. In Methods in Molecular Biology (Vol. 1763, pp. 29–42). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7762-8_4
Mendeley helps you to discover research relevant for your work.