Comparação entre dois protocolos para obtenção de plasma rico em plaquetas, em cães

Abstract

The objective of this paper was to analyze two protocols for the production of platelet rich plasma (PRP) in dogs. Peripheral blood of 20 adult dogs was collected into three tubes. The first was processed through protocol A - single centrifugation at 1200rpm for 10min -, the second was submitted to protocol B - a first centrifugation at 1200rpm for 10min and a second centrifugation at 1600rpm for 10min - and the third was used to perform platelet count in whole blood, which served as a parameter for values obtained in PRP. The protocol in which it was possible to achieve a higher platelet count was tested in other 20 dogs to evaluate its reproducibility. Protocol B resulted in a superior platelet count in 100% of the samples, concluding that the referred protocol is effective for PRP production in dogs.