Integrin expression in developing smooth muscle cells

Abstract

We studied the specific expression patterns and distributions of α1 and β1 integrin subunits, the major cell adhesion receptors in smooth muscle, in developing smooth muscle cells from 16-, 18-, and 20-day embryonic gizzards and from 1- and 7-day post hatch chick gizzards by SDS-PAGE, immunoblotting, and immunoelectron microscopy. Antibodies raised against α1 and β1 integrins isolated from avian gizzards were used as probes. Gels and blots showed that the amount of α1 and β1 integrins increased as age increased, with major increases at 1 and 7 days post hatch. Image analysis of immunoelectron micrographs demonstrated that statistically significant labeling increases occurred between embryonic Days 16 and 18, between embryonic Day 20 and 1 day post hatch, and between 1 day and 7 days post hatch. Immunolabeling with both anti-α1 and anti-β1 integrin was prominent at membrane-associated dense plaques (MADPs) and at filament anchoring regions at cell ends. This indicates that α1 and β1 integrin expression coincides temporally with the intra-cellular proliferation and reorientation of myofilaments. The similarity in distribution patterns of α1 and β1 integrins during development suggests that the two integrin subunits are synchronously expressed during development and do not appear sequentially.