Effect of 2,6-dichloroisonicotinic acid, its formulation materials and benzothiadiazole on systemic resistance to alternaria leaf spot in cotton

Abstract

A wettable powder (WP) formulation providing 5-25 μg mL-1 of 2,6-dichloroisonicotinic acid (INA) and 15-75 μg mL-1 of WP applied to cotton cotyledons significantly increased the resistance of the next two leaves to challenge inoculation by Alternaria macrospora. The wettable powder alone at 15-75 μg mL-1 had a lesser effect. A wettable granule (WG) formulation supplying 35 μg mL-1 of benzo-(1,2,3)-thiadiazole-7-carbothiotic acid S-methyl ester (BTH) and 35 μg mL-1 of WG, applied as a cotyledonary treatment, significantly reduced the formation of lesions on the subsequent two leaves when challenged with A. macrospora. The WG control had no effect. Each treatment except for the WG control also raised the activities of β-1,3-glucanase in unchallenged leaf and stem tissue. Each of the components of the wettable powder without INA applied to cotyledons rinsed enzyme activities in the next leaves. Individual components, as suspensions of silicic acid and kaolin and solutions of the detergent Attisol II, the wetting agent Ultravon W300 and pure INA, applied to cotyledons increased the resistance of the next leaves to A. macrospora. The responsiveness of cotton to BTH and to each of the components of formulated INA is discussed in relation to knowledge of the effects of BTH and INA on other plants and to possible ways in which the other components of the wettable powder may affect the process of signalling for systemic resistance to disease.