Effects of Various Phytochemical Fractions of Mundulea sericea on Free Radical Scavenging and Inhibition of Inflammatory Agents

Abstract

Antioxident and antiinflammatory activities of Mundulea sericea (Willd.) A. Chev. is a medicinally important species belong to the family Fabaceae. This species contains various phytocompounds of entire parts which act as antimicrobial activity, anti-inflammatory activity, anticancer activity, antidiabetic activity, etc. Hence, the present study was aimed to analysis of phytochemical constitutes through Fourier transform infrared (FTIR) and gas chromatography mass spectrometry (GCMS) analysis by six various solvent extracts of hexane (MSBH), petroleum ether (MSBPE), chloroform (MSBC), ethyl acetate (MSBEA), ethanol (MSBE), and methanol (MSBM). The result of phytochemical analysis reported that the MSBE and MSBM extracts are contain more phytochemical constitutes than other extracts. In FTIR and GCMS study, MSBE and MSBM extracts are reported various phytochemical constitutes and out that many bioactive compounds are present of these extracts. Further, MSBE extract was subjected antioxidant and anti-inflammatory activities. In antioxidant activity, four different type assays such as 2,2-diphenyl-1-picryl-hydrazyl-hydrate radical scavenging, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate), hydrogen peroxide, and nitric oxide (NO) were used. Among this antioxidant study, NO was reported 74.63 inhibition percentage of free radical than other assays. In anti-inflammatory activity, albumin denaturation and human red blood cell membrane stabilization assay were performed by MSBE extract which result reported that the MSBE extract has good anti-inflammatory activity with more than 60% inhibition capacity on both assays. From this study, M. sericea bark extracts contain highly therapeutic phytocompounds which compounds possess good antioxidant and inflammatory activities.