Intravital microscopy is a powerful tool that enables imaging various biological processes in live animals. Here, we describe a series of procedures designed to image subcellular structures, such as endosomes and secretory vesicles in the salivary glands (SGs) of live rats. To this aim, we used fluorescently labeled molecules and/or fluorescently tagged proteins that were transiently transfected in the live animal.
CITATION STYLE
Masedunskas, A., Sramkova, M., Parente, L., & Weigert, R. (2012). Intravital Microscopy to Image Membrane Trafficking in Live Rats (pp. 153–167). https://doi.org/10.1007/978-1-62703-056-4_9
Mendeley helps you to discover research relevant for your work.