Effect of Bacillus subtilis as probiotic on intestinal microbiota and growth performance of common carp (Cyprinus carpio)

Abstract

The current study was conducted to investigate the influence of dietary supplementation of Bacillus subtilis on the intestinal microbial community and growth performance profiles of common carp. The probiotic was isolated from local soils using PCR for amplification of 16S rDNA and added to the diet in a dose of ∼107 CFU g-1. The feeding trial was carried out for six weeks. A total of 64 fish (77.2 ± 0.86â g) were randomly divided into two groups: Control group (basal diet) and experimental group. The results of microbiology revealed that the resident total viable count (TVC) and lactic acid bacteria (LAB) were significantly decreased in the probiotic fed fish group compared to the control group, while the TVC in the digesta samples was significantly increased in the probiotic fed fish compared to the control group. The results of growth performance profiles were not influenced by probiotic diet. The results of the present study, demonstrate a potential effect for B. subtilis to modulate intestinal microbiota of common carp.