Determination of δ9-THC in whole blood using gas chromatography-mass spectrometry

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Abstract

A simple and reliable liquid-liquid extraction method for the determination of Δ9-tetrahydrocannabinol (THC) in whole blood utilizing gas chromatography-mass spectrometry in electron impact mode is described. The substance is derivatized with pentafluoropropionic anhydride in pentafluropropanol. The limit of detection is 0.5 ng/mL for a 1-mL specimen, with recovery greater than 70%. The intra-assay coefficient of variation (CV) is 3.1% to 5.2%, and the interassay CV is 6.4% to 9.5%, calculated at THC concentrations of 1,5, and 25 ng/mL. The accuracy is between 95 and 97%. The optimization of extraction and derivatization conditions is detailed.

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Chu, M. H. C., & Drummer, O. H. (2002). Determination of δ9-THC in whole blood using gas chromatography-mass spectrometry. Journal of Analytical Toxicology, 26(8), 575–581. https://doi.org/10.1093/jat/26.8.575

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