Identification and quantification of alkaloid compounds from different parts and production areas of Datura metel L

Abstract

An ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) method was used to identify alkaloids in six parts of Datura metel L. (D. metel L.). 65 Alkaloids were tentatively identified. Meanwhile, a rapid, sensitive and reliable ultra-high-performance liquid chromatography tandem triple quadrupole mass spectrometry (UPLC-Q-TRAP-MS/MS) technique had developed to determine the contents of 22 alkaloids in five minutes, with good precision and repeatability. This method provided a feasible solution for the content determination of alkaloid in D. metel L.