Human-induced pluripotent stem cells are known for their high proliferation capacity as well as their ability to differentiate to different lineages (Ban et al., Theranostics 7(7):2067–2077, 2017; Chen et al., Stem Cell Res 15(2):365–375, 2015; Serra et al., Trends Biotechnol 30(6):350–359, 2012). For stem-cell-derived cardiomyocytes to evolve into a scalable therapeutic source, a large quantity of highly pure cardiomyocytes is needed. Thus, lies the challenge of defining an efficient cardiomyocyte differentiation process. This chapter describes a method to evaluate multiple human-induced pluripotent stem cell lines for their cardiac differentiation potentials before evaluating their integrated proliferation and differentiation abilities in microcarrier cultures in a spinner culture format.
CITATION STYLE
Ho, V., Tong, G., Lam, A., Reuveny, S., & Oh, S. (2022). Integrating Human-Induced Pluripotent Stem Cell Expansion Capability and Cardiomyocyte Differentiation Potential in a Microcarrier Suspension Culture. In Methods in Molecular Biology (Vol. 2436, pp. 67–81). Humana Press Inc. https://doi.org/10.1007/7651_2021_423
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