Insilico Analysis of Novel Relb, Rele and Mazf Toxin-Antitoxin Homolog’s from the Genome of Xenorhabdus Nematophila

Abstract

Xenorhabdus nematophila is a motile gram-negative bacteria belonging to the family Enterobacteriaceae and is a natural symbiont of a soil nematode of family Steinernematidae. The bacterium is essential for effective killing of the insect host and is required by the nematode to complete its life cycle. X. nematophila can be grown under standard laboratory conditions and known to secrete several extracellular products, which include lipase(s), phospholipase(s), protease(s), and several broad spectrum antibiotics as the bacteria enter the stationary phase of their growth cycle and are believed to be secreted in the insect hemolymph. Recently, the genome of X. nematophila has been completely sequenced and annotated version is available in the NCBI database. In this study we have extensively analyzed bioinformaticaly the genome of X. nematophila with NCBI server (www.ncbi.nlm.nih.gov). Our results showed the presence of relB, relE, and mazF toxin-antitoxin homolog's at different loci in the genome. Later, various genes present in these loci were studied for phy-logenetic as well as physiochemical analysis. Promoter analysis of each module has been done to know the various tran-scription factors involved in their transcription.