Super-resolution microscopy enables the study of vaccinia architecture at subviral resolution with molecular specificity. Here, we outline how to use structured illumination microscopy (SIM) and stochastic optical reconstruction microscopy (STORM) to detect fluorescently tagged or immunolabeled viral proteins on purified virions. Tens to hundreds of individual virions can be imaged in a single field of view providing data for single-particle averaging or quantitative analysis of viral protein spatial organization.
CITATION STYLE
Gray, R., & Albrecht, D. (2019). Super-resolution Microscopy of Vaccinia Virus Particles. In Methods in Molecular Biology (Vol. 2023, pp. 255–268). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9593-6_16
Mendeley helps you to discover research relevant for your work.