Introduction: During a diagnostic evaluation of canine visceral leishmaniasis (VL), two of seventeen dogs were found to be co-infected by Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi. Methods: Specific polymerase chain reaction (PCR) and restriction fragment length polymorphism-PCR (RFLP-PCR) assays were performed. Results: PCR assays for Leishmania subgenus identification followed by RFLP-PCR analysis in biopsies from cutaneous lesions and the spleen confirmed the presence of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi in those fragments. Conclusions: This report reinforces the importance of using serological and molecular techniques in the epidemiological surveillance of canine populations in endemic areas in which both diseases are known to co-exist. In such cases, a reassessment of the control measures is required.
CITATION STYLE
Pires, M. Q., Madeira, M. de F., Bittencourt, V. R. E. P., & Pacheco, R. da S. (2014). Cutaneous and visceral leishmaniasis co-infection in dogs from Rio de Janeiro, Brazil: Evaluation by specific PCR and RFLP-PCR assays. Revista Da Sociedade Brasileira de Medicina Tropical, 47(2), 243–246. https://doi.org/10.1590/0037-8682-0007-2013
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