Rapid gynogenetic mapping of Xenopus tropicalis mutations to chromosomes

39Citations
Citations of this article
57Readers
Mendeley users who have this article in their library.

Abstract

Pilot forward genetic screens in Xenopus tropicalis have isolated over 60 recessive mutations. Here we present a simple method for mapping mutations to chromosomes using gynogenesis and centromeric markers. When coupled with available genomic resources, gross mapping facilitates evaluation of candidate genes as well as higher resolution linkage studies. Using gynogenesis, we have mapped the genetic locations of the 10 X. tropicalis centromeres, and performed fluorescence in situ hybridization to validate these locations cytologically. We demonstrate the use of this very small set of centromeric markers to map mutations efficiently to specific chromosomes. © 2009 Wiley-Liss, Inc.

Cite

CITATION STYLE

APA

Khokha, M. K., Krylov, V., Reilly, M. J., Gall, J. G., Bhattacharya, D., Cheung, C. Y. J., … Zimmerman, L. B. (2009). Rapid gynogenetic mapping of Xenopus tropicalis mutations to chromosomes. Developmental Dynamics, 238(6), 1398–1346. https://doi.org/10.1002/dvdy.21965

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free