THE concentration of Dorsal protein in the nucleus determines cell fate along the dorsoventral axis of the Drosophila embryo1-13. The dorsal-group genes and the cactus gene are required for production and transmission of a localized signal on the ventral side of the embryo4,5 which determines the position of the highest nuclear concentration of Dorsal protein1-3. The ventralizing signal produced in somatic cells6 is transmitted through the perivitelline space7 to the integral membrane protein Toll8. Inside the embryo it leads to dissociation of the cytoplasmic Dorsal-Cactus complex and subsequent nuclear localization of Dorsal protein9,10. Two components are known to mediate the signal transduction between Toll and Dorsal-Cactus11,12: Pelle, a serine/threonine protein kinase13, and Tube, a protein with an unknown biochemical activity14. Here we construct gain-of-function alleles of pelle and tube and show that pelle functions downstream of tube. In addition, Pelle and Tube interact directly with one another. We propose that Tube is a direct activator of the protein kinase Pelle.
CITATION STYLE
Großhans, J., Bergmann, A., Haffter, P., & Nüsslein-Volhard, C. (1994). Activation of the kinase Pelle by Tube in the dorsoventral signal transduction pathway of Drosophila embryo. Nature, 372(6506), 563–566. https://doi.org/10.1038/372563a0
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