Two-photon fluorescence microscopy allows three-dimensional imaging of biological specimens in vivo. Compared with confocal microscopy, it offers the advantages of deeper tissue penetration and less photodamage but has the disadvantage of slightly lower resolution.
CITATION STYLE
Mondal, P. P., & Diaspro, A. (2014). Multiphoton Fluorescence Microscopy. In Fundamentals of Fluorescence Microscopy (pp. 149–159). Springer Netherlands. https://doi.org/10.1007/978-94-007-7545-9_8
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