Multiphoton Fluorescence Microscopy

  • Mondal P
  • Diaspro A
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Abstract

Two-photon fluorescence microscopy allows three-dimensional imaging of biological specimens in vivo. Compared with confocal microscopy, it offers the advantages of deeper tissue penetration and less photodamage but has the disadvantage of slightly lower resolution.

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Mondal, P. P., & Diaspro, A. (2014). Multiphoton Fluorescence Microscopy. In Fundamentals of Fluorescence Microscopy (pp. 149–159). Springer Netherlands. https://doi.org/10.1007/978-94-007-7545-9_8

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