We present a protocol to combine behavioral recording and imaging using 2-photon laser-scanning microscopy in head-fixed larval zebrafish that express a genetically encoded calcium indicator. The steps involve restraining the larva in agarose, setting up optics that allow projection of a visual stimulus and infrared illumination to monitor behavior, and analysis of the neuronal and behavioral data.
CITATION STYLE
Orger, M. B., & Portugues, R. (2016). Correlating whole brain neural activity with behavior in head-fixed larval Zebrafish. In Methods in Molecular Biology (Vol. 1451, pp. 307–320). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3771-4_21
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