Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize

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Abstract

Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We construct a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2. Compared with previous reports in rice, we achieve much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors.

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Jiang, Y. Y., Chai, Y. P., Lu, M. H., Han, X. L., Lin, Q., Zhang, Y., … Chen, Q. J. (2020). Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize. Genome Biology, 21(1). https://doi.org/10.1186/s13059-020-02170-5

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