The readily programmable CRISPR-Cas9 system is transforming genome engineering. We and others have adapted the S. pyogenes CRISPR-Cas9 system to precisely engineer the Drosophila genome and demonstrated that these modifications are efficiently transmitted through the germline. Here we provide a detailed protocol for engineering small indels, defined deletions, and targeted insertion of exogenous DNA sequences within one month using a rapid DNA injection-based approach.
CITATION STYLE
Gratz, S. J., Harrison, M. M., Wildonger, J., & O’connor-Giles, K. M. (2015). Precise genome editing of drosophila with CRISPR RNA- guided cas9. Methods in Molecular Biology, 1311, 335–348. https://doi.org/10.1007/978-1-4939-2687-9_22
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