CENP-50 is required for papilloma development in the two-stage skin carcinogenesis model

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Abstract

CENP-50/U is a component of the CENP-O complex (CENP-O/P/Q/R/U) and localizes to the centromere throughout the cell cycle. Aberrant expression of CENP-50/U has been reported in many types of cancers. However, as Cenp-50/U-deficient mice die during early embryogenesis, its functions remain poorly understood in vivo. To investigate the role of Cenp-50/U in skin carcinogenesis, we generated Cenp-50/U conditional knockout (K14CreER-Cenp-50/Ufl/fl) mice and subjected them to the 7,12-dimethylbenz(a)anthracene (DMBA)/terephthalic acid (TPA) chemical carcinogenesis protocol. As a result, early-stage papillomas decreased in Cenp-50/U-deficient mice. In contrast, Cenp-50/U-deficient mice demonstrated almost the same carcinoma incidence as control mice. Furthermore, mRNA expression analysis using DMBA/TPA-induced papillomas and carcinomas revealed that Cenp-50/U expression levels in papillomas were significantly higher than in carcinomas. These results suggest that Cenp-50/U functions mainly in early papilloma development and it has little effect on malignant conversion.

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Saito, M., Kagawa, N., Okumura, K., Munakata, H., Isogai, E., Fukagawa, T., & Wakabayashi, Y. (2020). CENP-50 is required for papilloma development in the two-stage skin carcinogenesis model. Cancer Science, 111(8), 2850–2860. https://doi.org/10.1111/cas.14533

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