Dynamic mass redistribution assay decodes differentiation of a neural progenitor stem cell

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Abstract

Stem cells hold great potential in drug discovery and development. However, challenges remain to quantitatively measure the functions of stem cells and their differentiated products. Here, we applied fluorescent imaging, quantitative real-time PCR, and label-free dynamic mass redistribution (DMR) assays to characterize the differentiation process of the ReNcell VM human neural progenitor stem cell. Immunofluorescence imaging showed that after growth factor withdrawal, the neuroprogenitor stem cell was differentiated into dopaminergic neurons, astrocytes, and oligodendrocytes, thus creating a neuronal cell system. High-performance liquid chromatography analysis showed that the differentiated cell system released dopamine upon depolarization with KCl. In conjunction with quantitative real-time PCR, DMR assays using a G-protein-coupled receptor agonist library revealed that a subset of receptors, including dopamine D1 and D4 receptors, underwent marked alterations in both receptor expression and signaling pathway during the differentiation process. These findings suggest that DMR assays can decode the differentiation process of stem cells at the cell system level. © 2012 Society for Laboratory Automation and Screening.

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CITATION STYLE

APA

Pai, S., Verrier, F., Sun, H., Hu, H., Ferrie, A. M., Eshraghi, A., & Fang, Y. (2012). Dynamic mass redistribution assay decodes differentiation of a neural progenitor stem cell. Journal of Biomolecular Screening, 17(9), 1180–1191. https://doi.org/10.1177/1087057112455059

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