Molecular Cloning of Bovine CD14 gene

Abstract

Genomic DNA encoding bovine CD 14 was isolated from a bovine (Holstein) genomic library. Utilizing PCR fragment of mouse CD14 gene as a probe, we screened 9 × 105 plaques and obtained 3 clones containing the bovine CD14 gene. DNA sequencing showed that bovine CD 14 gene encodes 373 amino acids, and the coding sequence was separated by a 90 nt intron. The identity of the deduced amino acid sequence of bovine CD 14 was 61-73% to those of mouse, rabbit and human. Northern blot analysis revealed that CDI4 mRNA (1.5 kb) was expressed in the lung. Expression of CD14 mRNA was stimulated about 2-fold in bovine peripheral blood macrophage activated with LPS in vitro.