In vitro nematocidal activity of the Pleurotus djamor PdR-2 fraction against J2 of Meloidogyne enterolobii

Abstract

Recently, the root-knot nematode Meloidogyne enterolobii have generated important economic losses (65%) in worldwide agriculture. In the present study, the nematocidal activity of the PdR-2 fraction of Pleurotus djamor was evaluated against the second instar juvenile (J2) of M. enterolobii. Different concentrations of the PdR-2 fraction (0.039, 0.078, 0.156, 0.132, 0.625, and 1.25 mg mL-1) were evaluated, as well as the respective control groups (water and Levamisole, 5 mg mL-1) in a volume of 100 µL (n=4). J2s were exposed for 24 h and subsequently quantified, and the percentage of mortality was estimated. Data were analyzed by analysis of variance with the general linear model and a comparison of means with Tukey’s test (p < 0.05). The PdR-2 fraction at concentrations of 0.132, 0.625, and 1.25 mg mL-1 were significantly equal with respect to Levamisole application, showing a mortality of 87.6, 84.5, and 86.3%, respectively. At the lowest concentration (0.039 mg mL-1), 40.3% mortality was recorded, whereas 86% was recorded at the highest concentration evaluated (1.25 mg mL-1). The PdR-2 fraction of P. djamor had nematocidal activity against J2s of M. enterolobii.