Bimolecular fluorescence complementation with improved gateway-compatible vectors to visualize protein–protein interactions in plant cells

Abstract

The bimolecular fluorescence complementation (BiFC) assay is a powerful, flexible, and simple tool to study protein–protein interactions in living cells. To accelerate the production and assessment of BiFC constructs, Gateway-compatible multicolor BiFC vectors were generated to enable the simultaneous production of multiple fusion genes that have the split N- or C-terminal fragment of fluorescent protein with the gene of interest in a high-throughput manner. Two different transient expression techniques for the assessment of BiFC in plant cells are described.