Abstract
Bioinformatics approaches can identify sequence motifs associated with diguanylate cyclases (DGCs), but experimental demonstration of DGC enzymatic activity is often desired. This protocol describes a sensitive radioactive assay for DGC activity in which substrate and product are quickly and easily separated by thin-layer chromatography.
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APA
Kazmierczak, B. I. (2017). Determining diguanylate cyclase activity (radioactive assay). In Methods in Molecular Biology (Vol. 1657, pp. 285–289). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7240-1_22
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