Hybrid liposomes inhibit tumor growth and lung metastasis of murine osteosarcoma cells

Abstract

Antitumor effects of hybrid liposomes ( HL ) composed of l ‐α‐dimyristoylphosphatidylcholine ( DMPC ) and polyoxyethylene(23) dodecyl ether (C 12 (EO) 23 ) on the metastatic growth of murine osteosarcoma ( LM 8) cells were investigated in vitro and in vivo. Remarkable inhibitory effects of HL ‐23 on the growth of LM 8 cells were obtained through the induction of apoptotic cell death in vitro. It was also indicated that HL ‐23 should dramatically suppress the invasion of LM 8 cells and the formation of filopodia on the cell surface in vitro. Furthermore, significantly high therapeutic effects were observed in the homograft mouse models of LM 8 cells with lung metastasis after the treatment with HL ‐23 in vivo. That is, the histological analysis demonstrated that the primary tumor growth of LM 8 cells implanted subcutaneously into the mice was inhibited along with the induction of apoptosis. In addition, it was found that HL ‐23 significantly decreased the lung metastasis of LM 8 cells in the mouse models through the inhibition of primary tumor invasion. These results suggest that HL ‐23 could be a novel agent for the chemotherapy of osteosarcoma.