The gel mobility shift assay is a powerful technique for detecting and quantifying protein-RNA interactions. While other techniques such as filter binding and isothermal titration calorimetry (ITC) are available for quantifying protein-RNA interactions, gel shift analysis provides the added advantage that you can visualize the protein-RNA complexes. In the gel shift assay, protein-RNA complexes are typically separated from the unbound RNA using native polyacrylamide gels in Tris/borate/EDTA buffer, although an alternative Tris-glycine buffering system is superior in many situations. Here, we describe both gel shift methods, along with strategies to improve separation of protein-RNA complexes from free RNA, which can be a particular challenge for small RNA binding proteins. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Yakhnin, A. V., Yakhnin, H., & Babitzke, P. (2012). Gel mobility shift assays to detect protein-RNA interactions. Methods in Molecular Biology, 905, 201–211. https://doi.org/10.1007/978-1-61779-949-5_12
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