Hematopoietic stem cells (HSCs) have self-renewal activity and multipotency. Clonal analysis and determination of HSC differentiation potential into platelets and erythrocytes as well as leukocytes are essential for the study of self-renewal and lineage commitment in HSC. However, due to technical limitations, platelet and erythrocyte differentiation potentials have not been assessed. This chapter describes principles and methods for single-cell sorting, single-cell transplantation, and identification and quantitative analysis of cell contribution to platelets and erythrocytes in addition to leukocytes in mouse chimeras. © 2014 Springer Science+Business Media New York.
CITATION STYLE
Yamamoto, R., Morita, Y., & Nakauchi, H. (2014). Five-lineage clonal analysis of hematopoietic stem/progenitor cells. Methods in Molecular Biology, 1185, 237–245. https://doi.org/10.1007/978-1-4939-1133-2_16
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