EssD, a nuclease effector of the Staphylococcus aureus ESS pathway

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Abstract

Specialized secretion systems of bacteria evolved for selective advantage, either killing microbial competitors or implementing effector functions during parasitism. Earlier work characterized the ESAT-6 secretion system (ESS) of Staphylococcus aureus and demonstrated its contribution to persistent staphylococcal infection of vertebrate hosts. Here, we identify a novel secreted effector of the ESS pathway, EssD, that functions as a nuclease and cleaves DNA but not RNA. EssI, a protein of the DUF600 family, binds EssD to block its nuclease activity in the staphylococcal cytoplasm. An essD knockout mutant or a variant lacking nuclease activity, essDL546P, elicited a diminished interleukin-12 (IL-12) cytokine response following bloodstream infection of mice, suggesting that the effector function of EssD stimulates immune signaling to support the pathogenesis of S. aureus infections.

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Ohr, R. J., Anderson, M., Shi, M., Schneewind, O., & Missiakas, D. (2017). EssD, a nuclease effector of the Staphylococcus aureus ESS pathway. Journal of Bacteriology, 199(1). https://doi.org/10.1128/JB.00528-16

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