DNA polymerase α subunit residues and interactions required for efficient initiation complex formation identified by a genetic selection

Abstract

Background: A genetic selection revealed interactions important for replication initiation. Results: A PHP mutation ablated interaction with the ε subunit and distorted the active site. C-terminal mutations decreased binding by the clamp loader τ subunit. Conclusion: The β binding domain functions in both β and τ binding. Significance: This work advances our knowledge of replicase interactions critical for function.