The availability of whole genomic sequences provides a great framework for biologists to address a broad range of scientific questions. However, functions of most mammalian genes remain obscure. The forward genetics strategy of insertional mutagenesis uses DNA mutagens such as retroviruses and transposable elements; this strategy represents a powerful approach to functional genomics. A variety of methods to uncover insertion sites have been described. This chapter details SplinkTA-PCR and SplinkBlunt-PCR, modified from splinkerette-PCR, for mapping chromosomally the insertion sites of a murine leukemia virus that causes leukemia in the BXH-2 strain of mice. These protocols are easy to use, reliable, and efficient.
CITATION STYLE
Yin, B. (2011). Isolation of Genomic Insertion Sites of Proviruses Using Splinkerette-PCR-Based Procedures. In Methods in Molecular Biology (Vol. 687, pp. 25–42). Humana Press Inc. https://doi.org/10.1007/978-1-60761-944-4_3
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