Dengue virus photo-inactivated in presence of 1,5-iodonaphthylazide (INA) or AMT, a psoralen compound (4'-aminomethyl-trioxsalen) is highly immunogenic in mice

Abstract

Two novel methods of dengue virus inactivation using iodonaphthyl azide (INa) and aminomethyl trioxsalen (aMT) were compared with traditional virus inactivation by formaldehyde. The aMT inactivated dengue-2 virus retained its binding to a panel of 5 monoclonal antibodies specific for dengue-2 envelope protein, whereas inactivation by formaldehyde and INa led to 30-50% decrease in binding. all three inactivated viruses elicited high level virus neutralizing antibodies in vaccinated mice. However, only mice vaccinated with aMT inactivated virus mounted T cell responses similar to live, uninactivated virus. © 2013 Landes Bioscience.