Mixed Multiplex Allele-Specific PCR (MiMAS-PCR) test for rapid detection of MDR and XDR-TB from the sputum of pulmonary TB patients

1Citations
Citations of this article
28Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Until now, Multi-Drug Resistant TB (MDR-TB) and Extended Drug-Resistant TB (XDR-TB) are still challenging to detect rapidly, since the gold standard culture method takes at least eight weeks to achieve diagnostic results. In this research, we designed a rapid and accurate detection method to detect MDR and XDR-TB using a technique which identifies specific genes encoding susceptibility to first and second-line anti-TB drugs combined in a PCR technique called Mixed Multiplex Allele-Specific Polymerase Chain Reaction (MiMAS-PCR) directly from sputum specimens. From total 117 sputum samples, The MiMAS-PCR test demonstrated 98.4% and 100% specificity in MDR-TB and XDR-TB detection, respectively, but the sensitivity is only suitable for rapid detection of XDR-TB (100%), not for MDR-TB (43.9%). The MiMAS-PCR technique is suitable for the rapid detection of XDR-TB (in this study, only one sample), but not for MDR-TB from the sputum specimens. Other methods are required to identify MDR-TB rapidly for early treatment and to prevent spread to their surrounding contacts.

Cite

CITATION STYLE

APA

Massi, M. N., Subair, Hidayah, N., Wulandari, A. A., Abidin, R. S., Handayani, I., … Halik, H. (2019). Mixed Multiplex Allele-Specific PCR (MiMAS-PCR) test for rapid detection of MDR and XDR-TB from the sputum of pulmonary TB patients. In Journal of Physics: Conference Series (Vol. 1341). Institute of Physics Publishing. https://doi.org/10.1088/1742-6596/1341/7/072010

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free