Cloning and sequence of the gene for the cytoplasmic inogranic pyrophosphatase from the thermoacidophilic archaebacterium Thermoplasma acidophilum

Abstract

The gene (ppa) from the thermoacidophilic archaebacterium Thermoplasma acidophilum, encoding the cytoplasmic pyrophosphatase, has been cloned. Two degenerate oligonucleotide probes, synthesized according to the N‐terminal amino acid sequence of the isolated protein, were used to screen subgenomic libraries. The DNA‐derived amino acid sequence of the archaebacterial enzyme allows, for the first time, comparative studies of cytoplasmic pyrophosphatases to be extended to all three urkingdoms. The archaebacterial pyrophosphatase more closely resembles the eubacterial enzymes on the basis of sequence similarity and subunit size. The majority of amino acid residues considered to be essential for hydrolysis of pyrophosphate seem to have been conserved throughout evolution, as inferred from the results of an alignment of sequences from all three urkingdoms. Copyright © 1992, Wiley Blackwell. All rights reserved