Reverse zymography is a technique by which protease inhibitor(s) in a sample could be electrophoretically separated in a substrate-impregnated acrylamide gel and their relative abundance could be semi-quantified. The gel after electrophoresis is incubated with a protease when the impregnated substrate and all other proteins of the sample are degraded into small peptides except the inhibitor(s) that show clear bands against a white background. Since reverse zymography cannot distinguish between a protease inhibitor and a protein that is resistant against proteolysis, the results should be confirmed from inhibition of protease activity by solution state assay.
CITATION STYLE
Sharma, K., & Bhattacharyya, D. (2017). Reverse zymography: Overview and pitfalls. In Methods in Molecular Biology (Vol. 1626, pp. 125–132). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7111-4_11
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