Coaggregation of oral Bacteroides species with other bacteria: Central role in coaggregation bridges and competitions

Abstract

Seventy-three freshly isolated oral strains representing 10 Bacteroides spp. were tested for their ability to coaggregate with other oral gram-negative and gram-positive bacteria. None coaggregated with any of the gram-negative strains tested, which included Capnocytophaga gingivalis, C. ochracea, C. sputigena, and Actinobacillus actinomycetemcomitans. Strains of bacteroides buccae, B. melaninogenicus, B. oralis, and B. gingivalis failed to coaggregate with any of the gram-positive strains tested. However, six Bacteroides spp. coaggregated with one or more species of gram-positive bacteria. Most isolates of B. buccalis, B. denticola, B. intermedius, B. loescheii, B. oris, and B. veroralis coaggregated with strains of Actinomyces israelii, A. viscosus, A. naeslundii, A. odontolyticus, Rothia dentocariosa, or Streptococcus sanguis. The strongest coaggregations involved B. denticola, B. loescheii, or B. oris; 22 of 25 strains coaggregated with A. israelii. Only B. loescheii interacted with certain strains of S. sanguis; these coaggregations were lactose inhibitable and were like coaggregations between A. viscosus and the same strains of S. sanguis. In fact, B. loescheii and A. viscosus were competitors for binding to S. sanguis. Many bacteroides also acted as coaggregation bridges by mediating coaggregations between two noncoaggregating cell types (e.g., S. sanguis and A. israelii). Evidence for binding-site competition and coaggregation bridging involving noncoaggregating cell types from three different genera provides support for the hypothesis that these integeneric cell-to-cell interactions have an active role in bacterial colonization of the oral cavity.