The lux operon is a useful reporter for bioluminescence imaging due to its independence of exogenous luciferin supply, but its relatively low brightness hampers the imaging of single cells. This chapter describes a procedure for the imaging of individual Escherichia coli cells using an improved ilux operon. The enhanced brightness of ilux enables long-term bioluminescence imaging of single bacteria with high sensitivity without the requirement for an external luciferin.
CITATION STYLE
Gregor, C. (2020). Bioluminescent imaging of single bacterial cells using an Enhanced ilux operon. In Methods in Molecular Biology (Vol. 2081, pp. 43–52). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9940-8_4
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