The ultrasonic vocalization, or isolation calling, of infant rats and mice has been studied as a measure of anxious affective state and as an early communicative behavior between a pup and mother. The protocol described herein is the typical separation testing procedure. Also included are procedures used to modulate crying by providing contact with littermates and/or dam and increased isolation calling response by a prior brief maternal interaction. These procedures provide the basis for experimental research on the early development of emotion and communication in a critically important experimental model species - the mouse. © 2009 Humana Press.
CITATION STYLE
Winslow, J. T. (2009). Ultrasonic vocalizations by infant mice: An ethological expression of separation anxiety. Neuromethods. Humana Press. https://doi.org/10.1007/978-1-60761-303-9_5
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