Quality control of newly synthesized glycoproteins is tightly regulated by sugar processing of N-glycans and by recognition of specific glycan structures by lectins in the endoplasmic reticulum (ER). Mannose trimming and its recognition determine the targeting of misfolded glycoproteins for ER-associated degradation. ER degradation-enhancing α-mannosidase-like (EDEM) proteins in mammals and their homologue Htm1p/Mnl1p in Saccharomyces cerevisiae are involved in this process. To analyze the function of EDEM proteins, we expressed and purified recombinant EDEM3 from HEK293 cells and assessed its mannose-trimming activity in vitro.
CITATION STYLE
Hosokawa, N. (2020). In vitro mannosidase assay of EDEMs: ER Degradation-Enhancing α-Mannosidase-Like proteins. In Methods in Molecular Biology (Vol. 2132, pp. 151–158). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0430-4_15
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