New antibody derivatives are continuously being generated to interact with a range of therapeutic targets. The cost-effective and efficient production of these and other antibody derivatives is crucial for their further success. Here, we describe the construction of the expression vectors needed for heterologous expression of a Fab fragment in the yeast Pichia pastoris. The experimental conditions for lab-scale expressions are discussed, and an overview of an efficient purification strategy is presented. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Schoonooghe, S., Leoen, J., & Haustraete, J. (2012). Production of antibody derivatives in the methylotrophic yeast pichia pastoris. Methods in Molecular Biology, 907, 325–340. https://doi.org/10.1007/978-1-61779-974-7_19
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