Effect of novel drug (derived from curcumin and cefotaxime) on cefotaxime induced hepatorenal toxicity in rats

Abstract

Curcumin is important antioxidant and this due to the presence of β-diketone groups in its structure, it is the main components of turmeric extract from roots of curcuma longa L., it's a pigment with bright yellow color, curcumin have a wide range of therapeutic effects, chemistry it has two aromatic ring systems (phenols) that connected by α-β-unsaturated methylene groups with two carbonyl groups and these groups form stable enols that are ready to react. This study aimed to evaluate the possible protective effect of novel (drug derived from condensation of curcumin and cefotaxime) against cefotaxime induced hepato-renal toxicity in rats.Twenty-four rats were randomly divided into four equal groups; the first group (control group): Without any treatment. The second group (cefotaxime group) were injected intramuscular cefotaxime (90 mg/kg twice daily for seven days).The third group (novel drug) were received novel drug at dose 90 mg/kg twice daily for seven days. While the four group (cefotaxime +novel drug group) were received with cefotaxime at dose of 90 mg/kg plus novel drug 90mg/kg injected intramuscular twice daily along the experiment. Curcumin extracted from turmeric, cefatoxime was added to the solution of reaction(Schiff base), the orange mixture refluxed for 24h. the catalytic was glacial acetic acid few droops, in the end of reaction the solution cooled and then filtered, washed and dried, then recrystallization with ethanol. Cefotaxime produced significant increase in kidney function tested such as urea, creatinine, calcium, sodium and potassium as compared to control group. Also, cefotaxime caused an increase in liver function tests such as alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and total bilirubin. At the same time, cefotaxime caused increased in liver and kidney tissue MDA levels and decreased in liver and kidney tissue superoxide dismutase, catalase and glutathione peroxidase. However, these parameters were significantly ameliorated by the given of the novel drug. In conclusion, novel drug has protective effect against cefotaxime induced hepato-renal toxicity in rats. Novel drug reduced the side effects which produced by cefotaxime.