Abstract
Cell migration is a crucial step for wound healing. Assays able to evaluate cell migration are very useful to evaluate in vitro wound healing. Scratch wound assay creates a gap in confluent monolayer of keratinocytes to mimic a wound. The protocol of scratch wound is based on few steps: cell culture preparation, scratch wound assay, data acquisition, and data analysis.
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Martinotti, S., & Ranzato, E. (2020). Scratch wound healing assay. In Methods in Molecular Biology (Vol. 2109, pp. 225–229). Humana Press Inc. https://doi.org/10.1007/7651_2019_259
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