Dioxygen insensitive C70/AuNPs hybrid system for rapid and quantitative glucose biosensing

Abstract

A novel hybrid system based on NAD-dependent glucose dehydrogenase immobilized on gold nanoparticles (AuNPs) covered with C70 fullerene has been developed for effective biosensing and quantitative detection of glucose. According to amperometric measurements, the new biosensor exhibited high sensitivity (26.1 μA mM-1 cm-2), wide linear range (0.25-12.0 mM), and rapid characteristic response (3 s). These unique properties suggest that the prepared hybrid nanocomposite is very promising to find potential application as a dioxygen insensitive biosensor for rapid and quantitative detection of glucose.