Glycoprotein is a major element in higher organisms including mammalians and plants. It is widely accepted that variation in cellular N-glycome is related to modulation in dynamic cellular mechanisms such as cell-cell adhesion, cell activation, and malignant alterations in mammalian cells. However, the physiological importance of glycan modification of glycoproteins in plant cells is still a matter of dispute. Therefore, a comprehensive and high-throughput analysis of N-glycome in plant glycoproteins is needed. Here, an application of the glycoblotting-mass spectrometry technique to plant glycoprotein research is described. © 2014 Springer Science+Business Media, LLC.
CITATION STYLE
Kaneko, K., Shiraya, T., Mitsui, T., & Nishimura, S. I. (2014). Rapid and high-throughput N-glycomic analysis of plant glycoproteins. Methods in Molecular Biology, 1072, 645–653. https://doi.org/10.1007/978-1-62703-631-3_44
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