TaqMan® OpenArray® high-throughput transcriptional analysis of human embryonic and induced pluripotent stem cells

Abstract

It is widely accepted that somatic cells can be reprogrammed by a set of transcription factors to become embryonic stem cell-like: These reprogrammed cells, induced pluripotent stem cells (iPSCs), are nearly identical to embryonic stem cells (ESCs), because both have the capacity to self-renew and to form all cellular lineages of the body. Transcriptional differences between ESCs, iPSCs, and fibroblasts can be analyzed by quantitative PCR (qPCR) using TaqMan® Gene Expression assays, a widely used tool for rapid analysis of different cell types. In this chapter, we describe the OpenArray® platform which generates qPCR data from high-throughput instrumentation. We examined the gene signature profiles of ESCs, fibroblasts, and iPSCs with a TaqMan® OpenArray® Human Stem Cell Panel containing 631 TaqMan® Gene Expression assays that represent pathways involved in self-renewal, pluripotency, lineage patterning, transcriptional networks, stem cell differentiation, and development. © 2013 Springer Science+Business Media New York.