Simultaneous Determination of Hydrogen, Methane and Carbon Dioxide of Breath using Gas-Solid Chromatography

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Abstract

The analysis of respiratory hydrogen and methane was estimated as a useful index of intestinal fermentation of undigestible carbohydrate. A simultaneous and precious analysis of these gases as well as carbon dioxide was studied. A gas-impermeable multi-laminated film bag metalized by aluminum vapor was fitted to use as a storage bag; its impermeability was verified by measuring the residual rate of hydrogen after 3 months' storage. Hydrogen, methane and carbon dioxide of the breath gas even at 1 ppm could be determined simultaneously by using a gas-solid chromatography installed with a photoionization detector and active carbon column. To observe the genesis of hydrogen and methane after carbohydrate ingestion, pectin, a typical water-soluble dietary fiber, was fed to healthy volunteers. Increasing excretion of pulmonary hydrogen or methane showed the sign of intestinal fermentation as the results of carbohydrate malabsorption. © 1992, Center for Academic Publications Japan. All rights reserved.

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Tsuji, K., Shimizu, M., Nishimura, Y., & Nakagawa, Y. (1992). Simultaneous Determination of Hydrogen, Methane and Carbon Dioxide of Breath using Gas-Solid Chromatography. Journal of Nutritional Science and Vitaminology, 38(1), 103–109. https://doi.org/10.3177/jnsv.38.103

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