Patients with psychiatric disorders exhibit dysfunctions in peripheral and central metabolism. This may be a root cause of impaired neuronal function, manifested as changes in mood, behavior, and cognitive capabilities in patients suffering with these conditions. Here we describe a selective reaction monitoring mass spectrometry (SRM-MS)-based targeted proteomic protocol for precise simultaneous quantitation of three glycolytic enzymes in postmortem brain tissue extracts. The SRM-MS approach has several advantages in terms of sensitivity, reproducibility, and reduced sample consumption, compared to traditional MS methods.
CITATION STYLE
Lanfredi, G., Reis-de-Oliveira, G., Saia-Cereda, V. M., Guest, P. C., Martins-de-Souza, D., & Faça, V. M. (2017). Selective reaction monitoring mass spectrometry for quantitation of glycolytic enzymes in postmortem brain samples. In Advances in Experimental Medicine and Biology (Vol. 974, pp. 205–212). Springer New York LLC. https://doi.org/10.1007/978-3-319-52479-5_16
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